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Mcq on dna sequencing Sequencing is the sequence of occurrences of the four nucleotide bases i. This reaction is facilitated by the enzyme reverse transcriptase and the DNA thus formed is known as the complementary DNA or the cDNA. All the mRNA in an organism at a given time B. Illumina sequencing, a widely used method, employs fluorescently labeled nucleotides and reversible termination to sequence DNA fragments. 27 Multiple choice questions. Jan 11, 2025 · A genome is a complete set of DNA that contains all of the genes of an organism. This set of Molecular Biology Multiple Choice Questions & Answers (MCQs) focuses on “General Principles for Chain Termination Sequencing”. Which of the following statements are true regarding PCR a) billions of copies of desired DNA can be synthesized from microgram This set of Cell Biology Multiple Choice Questions & Answers (MCQs) focuses on “Gene Expression – Transcription”. c) Both strands of DNA are arranged parallel. The Test: DNA & RNA MCQs are made for NEET 2025 Exam. com. Understand the sequencing methodologies, technologies, and applications in Biotechnology, Genetic Engineering and Genomics. (C) The guide RNA binds to a random DNA sequence, and the Cas9 protein deletes the DNA at that location. View Answer & Explanation Category: Biotech Engineering MCQs Sub Category: Genetic Engineering Mcqs This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Basic Local Alignment Search Tool (BLAST)”. Nov 8, 2024 · Option 1 (RNA primer, dNTPs, buffer, primase, DNA template): This combination is more suited for DNA replication, not reverse transcription. Which of the following statements is accurate for the PCR – polymerase chain reaction? (a) Automated PCR machines are called thermal cyclers The DNA Sequencing MCQ with Answers PDF (DNA Sequencing Multiple Choice Questions (MCQs) PDF e-Book) download Ch. Next Generation Sequencing MCQ $. PCR is a a) DNA degradation technique b) DNA amplification technique c) DNA sequencing technique d) all of these, 3. ) Maxam gilbert (no longer used) 2. Introduction to Computational Biology MCQ $. Any DNA molecule that has the ability to replicate in an appropriate host cell, to which the desired gene are integrated for cloning, is called as a) Plasmid b) linker c) vector d) adapter, 2. It usually is a singular circular molecule c. Figure: Palindrome nucleotide sequence So, the correct answer is option 2. What Is Genome Sequencing? A genome is a complete set of genetic instructions which are present in an organism in its DNA. Option 2 (DNA primer, NTPs, buffer, DNA template): NTPs (ribonucleotide triphosphates) are needed for RNA synthesis, not DNA synthesis. 2) Phosphodiester bonds are cleaved by nucleases a group of enzymes present in the cells. This force can stabilize a DNA double-helix (a) Hydrophilic sugar-phosphate groups are found on the exterior of the helix where interaction with water occurs This set of Class 12 Biology Chapter 6 Multiple Choice Questions & Answers (MCQs) focuses on “The Genetic Material is DNA”. Study with Quizlet and memorize flashcards containing terms like Western blotting is the technique for the detection of a) specific DNA in a sample b) specific RNA in a sample c) specific protein in a sample d) specific glycolipid in a sample, 2. Pick the odd one out. #basicdnastructure #dnasequencing #whereisdnafound Study with Quizlet and memorize flashcards containing terms like Which of the following correctly describes the shapes of the major and minor grooves in double-helical RNA? The major groove is narrow and deep, The underlying principle of "classical" methods of DNA sequencing is to separate, by size, nested sets of DNA molecules. a) True b) False View Answer Test: DNA & RNA for NEET 2025 is part of NEET preparation. Which of the following is incorrect about a microarray? a) It is a slide attached with a high-density array of immobilized DNA oligomers representing the entire genome of the species under study Feb 27, 2021 · Multiple Choice Questions (MCQs) 1. Also, the template MCQ on DNA Sequencing https://www. The identification of drugs through the genomic study is called_____. Whether you’re studying for an exam or seeking to deepen your understanding of molecular genetics, these MCQs offer valuable learning opportunities for Jun 17, 2023 · Which one of the following is NOT true regarding DNA? a) DNA is double-stranded. 0 International (CC BY 4. bonds in a sequence. html Happy Learning Class 12 Biology MCQ – Molecular Basis of Inheritance – Methodologies of HGP ; Genetic Engineering Questions and Answers – Expression of Coding Section in-Vitro ; Molecular Biology Questions and Answers – Most Mobile DNA Consists of Transposable Elements ; Class 12 Biology MCQ – Molecular Basis of Inheritance – Salient Features of HGP Jan 1, 2022 · MCQ on DNA sequencing https://www. The BLAST program was developed in _____ a) 1992 b) 1995 c) 1990 d) 1991 View Answer 4. DNA Questions - Khan Academy the array of the elements of purified principle agreed closely with DNA and the principle centrifuged to the same level as DNA. Inside the nucleus B. The section contains bioinformatics multiple choice questions and answers on heuristic database searching, blast, fasta, smith – waterman method, fasta and blast comparison. The entire substrates Biology Multiple Choice Questions and Answers for Different Competitive Exams obtaining double stranded copies of cloned DNA suitable for DNA sequencing. Dec 16, 2024 · Transcription is the first step in gene expression, where the DNA sequence of a gene is transcribed into RNA. html Lear more: What is DNA Sequencing? Sanger Dideoxy Dec 14, 2023 · In prokaryotes, the process of removing and replacing RNA primer from the lagging strand during DNA replication is carried out by an enzyme called DNA polymerase I. . Scientists and Discoveries in Genomics MCQ $. B) Three dimensional structure of a protein is essential for correct function, and the process of microarray preparation may interfere with this structure. In this section, you'll find a wide range of DNA Sequencing and DNA Replication mcq questions that explore various aspects of DNA Sequencing and DNA Replication problems. b) The object of DNA cloning is to amplify DNA. With other cellular contents C. It is supported by automated DNA sequencing methods and computer software to assemble the massive sequence data. Which of the following is a chemical nucleotide sequencing method? 3. The Test: DNA & RNA questions and answers have been prepared according to the NEET exam syllabus. Question 1: For analyzing DNA sequences, two techniques have been developed, one is _____ method frequently termed_____, involving Quiz yourself with questions and answers for Sanger Method of DNA Sequencing - practice test, so you can be ready for test day. (A) The guide RNA binds to a specific DNA sequence, and the Cas9 protein cuts the DNA at that location. In which year, the 3-D structure of DNA was introduced? A Oct 27, 2024 · Get Sequencing Multiple Choice Questions (MCQ Quiz) with answers and detailed solutions. genetic fingerprinting Practice questions, MCQs, Past Year Questions (PYQs), NCERT Questions, Question Bank, Class 11 and Class 12 Questions, NCERT Exemplar Questions and PDF Questions with answers, solutions, explanations, NCERT reference and difficulty level Class 12 Biology MCQ – Biotechnology – Processes of Recombinant DNA Technology – 1 ; Cell Biology Questions and Answers – Techniques – Polymerase Chain Reaction ; Class 12 Biology MCQ – Biotechnology – Processes of Recombinant DNA Technology – 2 ; Class 12 Biology MCQ – Tools of Recombinant DNA Technology: Cloning Vectors Attempt Techniques MCQ - 21 questions in 50 minutes - Mock test for IIT JAM preparation What is the sequence of the DNA molecule in question. ) DNA sequencing quiz for 12th grade students. html This set of Molecular Biology Multiple Choice Questions & Answers (MCQs) focuses on “Most Mobile DNA Consists of Transposable Elements”. 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DNA (Deoxyribonucleic acid) is the molecule that carries the genetic instructions used in growth, development, functioning, and reproduction of all known living organisms. 6. The palindrome DNA sequence is the sequence of bases that reads the same on the two strands when the orientation of the reading is unchanged. The first significant DNA sequence to be obtained was that of ________ MCQ on DNA Sequencing Principles and Applications. 5. b) The nucleotides are covalently linked by 3'-5' phosphodiester bond. html Happy Learning PCR and Restriction Fragment Length Polymorphism are the methods for 1. The seven important methods used for DNA sequencing are: (1) Sanger’s Method (2) Maxam and Gilbert Method (3) Hybridization Method (4) Pal Nyren’s Method (5) Automatic DNA Sequencer (6) Slab Gel Sequencing Systems and (7) Capillary Gel Electrophoresis. RFLP is used to (a) DNA probes (b) DNA polymerase (c) DNA microarrays (d) DNA fingerprinting. Supercoiling c. This force can stabilize a DNA double-helix (a) Hydrophilic sugar-phosphate groups are found on the exterior of the helix where interaction with water occurs MCQ for DNA sequencing. Sequencing of genomes depends on the assembly of a large number of DNA reads into a linear, contiguous DNA sequence. Now a days there are technologies available for sequencing on double stranded DNA, thus it reduces the use of M13 vectors these days. DNA staining is done by and more. Illumina Sequencing of DNA MCQ $. This set of Cell Biology Multiple Choice Questions & Answers (MCQs) focuses on “Nucleic-acid Techniques”. Antibodies recognize relatively short peptide sequences from a protein, and presence of the same peptide sequence in several proteins is possible. Which of the following is used to lyse the nucleus and release the DNA? a) sodium dodecyl sulfate b) ammonium sulfate c) ferric phosphate d) fluorine View Answer MCQ on DNA Sequencing https://www. 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Dec 31, 2024 · In next-generation sequencing (NGS), what is the purpose of a “read”? a) A short segment of RNA that binds to a template b) A segment of DNA generated during the sequencing process c) A unique identifier for each sequencing experiment d) A data file containing raw sequencing results. (a) It is a DNA sequencing technique. It uses a DNA template instead of an RNA template. Find important definitions, questions, notes, meanings, examples, exercises, MCQs and online tests for Test: DNA & RNA below. The technique used to identify specific DNA sequence in bacterial colonies is a) colony hybridization b) in situ hybridization c) dot blot technique d) western blotting 2. Dec 31, 2021 · Latest genome sequencing in Mumbai finds one-third of samples positive for Omicron. 5. These questions are beneficial for various competitive exams, placement interviews, and entrance tests. 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Each MCQ is crafted to challenge your understanding of DNA Sequencing and DNA Replication principles, enabling you to refine your problem-solving techniq DNA Sequencing, Mutation and Repair MCQ Questions and Answers based on the Microbiology for interview, preparation of competitive exams and entrance test Dec 31, 2024 · c) To direct the Cas9 protein to a specific DNA sequence d) To provide a template for new DNA synthesis. Click on “WhatsApp” to share this quiz. Here we mentioned some of areas. 1962. 0. DNA Sequencing gives knowledge about organism’s genetic sequence that can be used in a number of areas. Inside the ribosomes D. Real Time PCR. Coli – 4 MCQ on Bioinformatics: Concept and Applications. What are the viruses that affect bacteria known as? a) Virophage b) Bacteriophage c) Mimivirus d) Virology View Answer This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Protein – Protein Interactions”. 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D Entrance 1. a) Introns b) Cassettes c) Jumping genes d) Insertion sequences View Answer This set of Cell Biology Multiple Choice Questions & Answers (MCQs) focuses on “Genome Sequencing”. 2. d) Enhancing RNA polymerase activity Jun 4, 2022 · Multiple Choice Questions & Answers (MCQs) focuses on “Molecular Basis of Inheritance – DNA Fingerprinting”. B) protein digesting enzymes did not affect the sample: C) the extraction of lipids and proteins from the principle only slightly reduced its activity: D) DNA-digesting enzyme, DNase, destroyed all transcription activity. B. Both . This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Applications”. mcqbiology. study of enzymes 2. c) The object of PCR is to amplify DNA d) The object of DNA sequencing is to amplify DNA Question 3. 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In the beads on a string model, the bead is made up of _____ a) 6 histone proteins b) 8 histone proteins c) 6 histone proteins and DNA d) 8 histone proteins and DNA View Answer a) restriction Fragment Length Polymorphism (RFLP) b) Random amplified Polymorphic DNA (RAPD) c) Amplified Fragment Length Polymorphism (AFLP) d) Simple Sequence repeats (SSR) 5. DNA Sequencing is the process of determining the nucleic acid se This MCQ set consists of Molecular Biology Multiple Choice Questions from the topic Structure and Functions of DNA (PG Level) with Answer Key. Answer: c) To direct the Cas9 protein to a specific DNA sequence Explanation: The guide RNA in the CRISPR-Cas9 system is designed to match a specific DNA sequence in the genome. The formation of Oct 15, 2024 · Do you know all about DNA and RNA? Check out this molecular Biology MCQ quiz with answers and review your knowledge of DNA and RNA structure, replication, transcription & translation. genetic transformation 3. The _____ resolution genome map is the genomic DNA sequence that can be considered as a type of ______ map describing a genome at the single base-pair level. Which of the following does not affect the formation of hybrid DNA? a) Ionic strength b) Pressure c) Temperature d) Homologous DNA View Answer This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “Protein Sequences and Evolution”. The enzyme used in Maxam-Gilbert method for 32P labelling of the DNA at 3’ end is. Through iterative cycles of nucleotide incorporation and imaging, it accurately determines the sequence, offering high throughput, speed, and cost-effectiveness, revolutionizing genomic research and applications. (B) The CRISPR RNA binds to a specific DNA sequence, and the Cas9 protein repairs the DNA at that location. The first recombinant DNA molecule was synthesized in the year _____ A. Click on the “Submit” button when done. « Prev - DNA Topology Test – 3» Next - DNA Sequencing Test – 2 Take a quick interactive quiz on the concepts in Next-Generation DNA Sequencing Techniques or print the worksheet to practice offline. 26-52 to prepare MCAT Biology Practice Tests. DNA sequencing is done by a) Maxam Gilbert method b) Sanger dideoxy method c) Both a and b d) Watson and Crick, 2. It involves a series of enzymatic reactions, generating light proportional to the number of incorporated nucleotides, allowing for accurate sequence determination. Shotgun sequencing is a method to sequence DNA by randomly breaking it into smaller fragments, sequencing them, and then assembling the overlapping sequences. Prokaryotes B. Jan 8, 2025 · A nanopore is a DNA sequencing technique that helps in determining the exact sequence of DNA. The property of complementary nucleic acid sequences is to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. a. It provides insights into genetic variations, mutations, and diseases, aiding in personalized medicine, evolutionary studies, and understanding genetic traits and predispositions. MCQ on DNA Sequencing https://www. Which of the following is useful in applications of PCR? a) It is manual b) Only one sample’s analysis can be carried out at a time c) It is having a high speed d) The amount of DNA required initially is high View Answer Study with Quizlet and memorize flashcards containing terms like Which of the following statements best describes the genome of an organism? A. 3. The physical contacts between domains are crucial for the functioning of the cellular machinery. a) Initial DNA sequencing reactions generate short sequence reads from DNA clones b) To assemble a whole genome sequence, these short fragments are joined to form larger fragments c) The average length of the reads is about 50 bases d) A number of overlapping contigs can be further merged to form scaffolds View Answer 1. This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “DNA & Genomic Sequencing”. 1992. D. Site directed mutagenesis. It tests knowledge on techniques used to isolate DNA from plant and bacterial cells, including reagents like CTAB, lysozyme, phenol, and ethanol precipitation. 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The DNA fingerprinting involves identifying differences in some specific regions in DNA sequence called as (a) non repetitive DNA (b) repititive DNA (c) Exons (d) all the above This set of Verbal Reasoning Questions and Answers (MCQs) focuses on “Sequencing”. 1982. In the Sanger method of DNA sequencing, what causes the termination of chain elongation? Which of the following statements regarding the polymerase chain reaction is untrue? Why are DNA polymerases from thermophilic organisms used in the polymerase chain reaction? This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Genome Sequencing”. Plasmids . Genome sequencing involves figuring out the order of bases in an organism's entire genome. This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Genetics – The Polymerase Chain Reaction and DNA Sequencing”. DNA sequencing followed by genome annotation are steps of . What are the differences in the specific regions of DNA sequence called during DNA finger printing? This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Sequence Assembly and Gene Identification – 1”. This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “DNA Cloning: The Basics-1”. used often in dna sequencing, lacks a(n) Jun 4, 2022 · Principle of DNA Microarray Technique The principle of DNA microarrays lies on the hybridization between the nucleic acid strands. The whole-genome shotgun sequencing approach depends primarily on: A) rapidly sequencing thousands of small randomly cloned fragments: B) methodical sequencing a few large cloned fragments of DNA: C) sequencing the bacterial chromosome while it is still intact: 9: The goal of _____ is to determine the location of specific genes within the Mar 17, 2024 · DNA sequencing is a laboratory technique used to determine the precise order of nucleotides (A, C, G, T) in a DNA molecule. The questions cover cell lysis methods, roles of different treatments, and the correct order of extraction steps. Study Recombinant DNA and Biotechnology Test PDF, DNA Sequencing Multiple Choice Questions (MCQ Quiz) to apply to colleges online. Shotgun Sequencing of DNA MCQ $. Status Bar. All the proteins in an organism at a given time C. These short objective type questions with answers are very important for Board exams as well as competitive exams of Molecular Biology, Biotechnology, Genetics etc. Term. htmlWhat is DNA Sequencing? Sanger Dideoxy Se Upon comparison, the mRNA is found to contain 1,000 fewer bases than the DNA sequence. Which of the following technique is used in DNA finger printing? a) Western blotting b) Southern blotting c) Northern blotting d) Eastern blotting, 3 Oct 24, 2024 · A codon is a sequence of three DNA or RNA nucleotides that corresponds with a specific amino acid or stop signal during protein synthesis (Translation). com/2015/02/multiple-choice-questions-on-dna. Both D. Answer: C. This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Microarray-Based Approaches”. DNA Sequencing MCQ $. Ribonuclease protection assay, Gel retardation assay, DNA foot printing, DNA finger printing, DNA profiling. 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(a) Genomics (b) Pharmacogenomics (c) Pharmacogenetics (d) Cheminformatics. Eukaryotes C. The advent of rapid DNA sequencing methods has greatly accelerated biological Q1) Multiple Choice Questions a) DNA sequencing is done by 1) Maxam Gilbert method 2) Sanger dideoxy method 3) Both 1 and 2 4) Watson and Crick b) A vector should have which of the following properties i) MCS ii) Small size iii) Multiple ori iv)low replication speed 1) i, ii, iii 2) ii, iii, iv 3) i, ii, iv 4) i, iii, iv This set of Bioinformatics Multiple Choice Questions & Answers (MCQs) focuses on “Comparison of SAGE and DNA Microarrays”. Which of the following is untrue about SAGE? a) This approach is much more efficient than the EST analysis b) This approach is quite less efficient than the EST analysis MCQ on DNA Sequencing https://www. C. What is ‘X’? NEET MCQ on DNA Structure PDF:-Download the PDF Here. Rajendra Chavhan, Department of Zoology, RMG College, Nagbhid 1. 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Purpose: Identifies the exact nucleotide sequence of DNA fragments. Answer: B. DNA serves as a genetic material for which type of cell? A. ) Sanger Sequencing 3. It is mostly focused on nucleic acids (DNA and RNA) and proteins. viruses . Which of the following is not required for DNA sequencing? a) Restriction digestion b) Electrophoresis c) Cloning d) Polymerase chain reaction View Answer Next-generation sequencing (NGS) encompasses advanced DNA sequencing technologies capable of high-throughput, parallel sequencing of millions of DNA fragments. (b) It is a DNA degradation technique (c) It is a DNA amplification technique (d) All of the above. It reveals the genetic code and is essential for understanding genes, genetic variations, and numerous applications in genetics and biology. Short stretches of DNA used to identify complementary sequence in a sample is called probes markers VNTRs minisatellites 8. For RFLP A, you can detect either a 5,000bp band or a 3,350bp band; for RFLP B you can detect either a 4,300bp band or a pair of bands of 2,300 and 2,000bp. Pyrosequencing of DNA MCQ $. Not present. b) Structural genomics. These objective type questions are very important for competitive exams like NEET, AIIMS, JIPMER etc. html Lear more: What is DNA Sequencing? Sanger Dideoxy ADVERTISEMENTS: This article throws light upon the seven important methods used for DNA sequencing. Sol: (b) Pharmacogenomics. It guides the Cas9 protein to the correct location, where it Jun 10, 2022 · 3. c Vector Biology and Gene Manipulation MCQ (Multiple Choice Questions) Gene Manipulation Questions and Answers – Gene Manipulation in Gram – Negativ… Molecular Biology Questions and Answers – Construction of DNA and RNA Libraries ; Vector Biology Questions and Answers – Cloning Vectors for E. Some genes are dominant to others d. 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It is located in the nucleoid b. The DNA Sequencing MCQ with Answers PDF: DNA sequencing is used in; for questions to ask during an interview. BLAST MCQ $. This is an Jan 20, 2025 · DNA sequencing: Chemical and enzymatic methods. Which of the following is used in recombinant DNA technology? A. Chain-termination is a type of ______________ Explanation: One of the commonest methods of sequencing is the Sanger sequencing which is also known as chain-termination. Molecular biology is a branch of Biology that is concerned with studying chemical structures and biological phenomenon processes. Automated sequencing uses _____________ a) Enzymatic process b) Radiolabelling c) Chain termination d) Fluorescent Jun 23, 2020 · This Video contains most important questions about DNA Sequencing and it's different methods. Sol:(c) It is a DNA amplification technique. Such DNA produce will not be exactly same as its parent gene as it will lack the introns as it is converted from the RNA. Which of the following does not act as a restriction enzyme? a) EcorI b) BamHI c) HindIII d) polydeoxyribonucleotide synthase View Answer Sanger Sequencing Sanger Sequencing: A DNA sequencing method developed by Frederick Sanger in the 1970s. Where is DNA present in the eukaryotic cells? A. Which of the following compounds has desirable properties to The document presents 10 multiple choice questions about DNA extraction methods and protocols. All the introns in the nuclear DNA of an organism. Author: Dr. PCR: Types and applications. Download these Free Sequencing MCQ Quiz Pdf and prepare for your upcoming exams Like Banking, SSC, Railway, UPSC, State PSC. In this section, you'll find a wide range of DNA and Genomic Sequencing mcq questions that explore various aspects of DNA and Genomic Sequencing problems. Which of the following is untrue about DNA sequencing methods? a) Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR) Interactive MCQs on “DNA Sequencing”: Solve the following 10 questions. 9. MCQss. There are two main steps involved: (1) running a PCR-like b) It provides a series of forms that can be filled out to retrieve a Medline reference related to the molecular biology sequence databases c) One straightforward way to access the sequence databases is through ENTREZ d) It provides a series of forms that can be filled out to retrieve a DNA or protein sequence View Answer a) used to generate single stranded copies for DNA sequencing b) used to generate double stranded copies for DNA sequencing c) both a and b d) none of these 13 . cbyqky abhwsw qktradpgq lntbn uoder trocsj ocygp aanbk pljyl orfv